Daily Stress and Heart Rate Variability Among Mindfulness Meditation Practitioners: mHealth Observational Study

Clinical Snapshot

Key Findings

• P Value: Kruskal-Wallis P=0.02 for between-group stress differences; P<0.001 for runner vs control HRV

• Effect Size: Acute RMSSD increase of +4.68ms during meditation; stress-associated RMSSD reduction of -2.24ms

• Primary Outcome: Heart rate variability (RMSSD) and subjective stress levels

• Nnt Or Sensitivity: Effect maintained for at least 30 minutes post-meditation

• Confidence Interval: 95% CI 2.96-6.38ms for meditation effect; 95% CI -3.97 to -0.26ms for stress effect

Clinical Application

Requires smartphone app and compatible smartwatch; may be suitable for digital health interventions Could inform TGA-approved digital therapeutics; relevant to RACGP stress management guidelines and potential PBS digital health initiatives Adults interested in stress management through mindfulness meditation

Abstract

BACKGROUND: Mindfulness meditation has been reported to reduce stress and enhance well-being. However, its effects on heart rate variability (HRV)-a physiological marker of stress-remain underexplored. OBJECTIVE: This study aimed to examine how meditation practice is associated with subjective stress, HRV, and their interaction, using mobile health technologies. METHODS: This 3-week observational study included 90 participants-19 meditation practitioners (meditation group), 32 recreational runners as an active control group characterized by lower stress and higher HRV (running group), and 39 individuals without regular meditation or exercise habits (control group). HRV was continuously recorded using Garmin smartwatches. Subjective stress levels and activity states were assessed 3 times daily through a smartphone-based experience sampling method, yielding a total of 4557 responses (mean 50.6, SD 22.8 per participant). From the meditation group, start and end times of 632 daily meditation sessions (mean 33.3, SD 18.3 per participant) were also collected via the app. Standardized questionnaires on stress and related measures were administered at the end of the study period. RESULTS: The questionnaire survey confirmed that stress levels were significantly lower in both the meditation and running groups compared with controls (median Perceived Stress Scale scores: meditation 21, IQR 17-24; running 22, IQR 19-25; control 25, IQR 21-30; Kruskal-Wallis P=.02; adjusted Wilcoxon P=.05 and .04, respectively). Smartwatch-derived HRV (root-mean-square of successive differences [RMSSD]) was elevated in the running group relative to controls (median 47.0, IQR 44.0-54.2 and median 42.0, IQR 34.2-47.8, respectively; P<.001), whereas no significant difference was observed between the meditation and control groups (median 40.8, IQR 35.5-44.4 and median 42.0, IQR 34.2-47.8, respectively). Bayesian analysis of the experience sampling method data indicated that higher subjective stress levels were associated with a concurrent RMSSD reduction of -2.24 (95% credible interval -3.97 to -0.26) milliseconds. Although this pattern was consistent across groups, the steeper decline of -3.94 (95% CI -7.04 to -0.74) milliseconds was observed only in the running group, likely reflecting their elevated baseline HRV. Additionally, Bayesian modeling of 632 logged meditation sessions revealed an acute RMSSD increase of +4.68 (95% CI 2.96 to 6.38) milliseconds during meditation, with effects maintained for at least 30 minutes post practice. CONCLUSIONS: Although HRV among meditation practitioners did not appear elevated in overall daily life, the ability to increase HRV at arbitrary timings, along with the prolonged residual effect, may correlate with stress reduction. This hypothesis requires further exploration with adequate controls. Despite the preliminary nature of this study due to its limited sample size, our findings highlight the potential of mobile health-based methodologies to capture stress and HRV dynamics in real-world settings.

References

1. 1.Takezawa, J., Geng, S., Fujino, M., Miyake, M., Sasahara, K., Yatani, K., & Niida, A. (2026). Daily stress and heart rate variability among mindfulness meditation practitioners: mHealth observational study. Journal of Medical Internet Research, 28(5), e78244. https://doi.org/10.2196/78244